For research use only.

Applications: IHC, IF  

Species Cross-Reactivity: H, M, R, Fruit Fly, Nematode     

Molecular Weight: ~220KD     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: IHC: 1:200 IF: 1:100

Optimal dilutions should be determined by the end user.

Specificity: The Myosin Heavy Chain Mouse Monoclonal antibody detects endogenous MHC proteins 

Background: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali

light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms

in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts

in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the

alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with

several different dilated and hypertrophic cardiomyopathies..

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                          ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep