For research use only.

Applications: WB, IHC  

Species Cross-Reactivity: R

Molecular Weight: ~45KD     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:2000-5,000 IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The GFAP Mouse Monoclonal antibody detects endogenous GFAP proteins. 

Background: GFAP is a member of the class III intermediate filament protein family. It is heavily, and

specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite

cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural

stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of

astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily

express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary

tumors and has been reported in erythrocytes..

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                           ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep