For research use only.

Applications: IHC     

Species Cross-Reactivity: H,M,R   

Molecular Weight: N/A       

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The MAP2 Mouse Monoclonal antibody detects endogenous MAP2 proteins. 

Background: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of

MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the

third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are

present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time,

the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is

completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin

D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels

in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form

side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                          ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep