For research use only.

Applications: IHC  

Species Cross-Reactivity: H,M,R    

Molecular Weight: ~51KD     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions:  IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The CK16 Mouse Monoclonal antibody detects endogenous CK16 proteins 

Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the

suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal

breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show

diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas

immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these

markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer

may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor)

cells are not proliferating.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                              ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep