For research use only.

Applications: WB,

Species Cross-Reactivity: H, M, R,Pg     

Molecular Weight: ~70KDa

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:1,000-2,000

Optimal dilutions should be determined by the end user.

Specificity: HSP70 Mouse monoclonal antibody detects endogenous HSP70 proteins. 

Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat

shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an

important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is

usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that

spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the

ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid

residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state

Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding

domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                        ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep