For research use only.

Applications: WB, IP, IF

Species Cross-Reactivity: N/A

Molecular Weight: N/A

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000   IP:1:200  IF:1:2,000

Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins..

Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not

appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been

used extensively as a general epitope tag in expression vectors. It can be used for affinity

chromatography, then used to separate recombinant, overexpressed protein from wild-type protein 

 expressed by the host organism. It can also be used in the isolation of protein complexes with multiple

subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is

no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein

with an antibody against the Flag sequence.