For research use only.

Applications: WB, IHC

Species Cross-Reactivity: H

Molecular Weight: ~147KDa

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:2,000  IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The CD45 Mouse Monoclonal antibody detects endogenous CD45 proteins 

Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four

isoforms which share a common large intracellular domain. Their extracellular domains are heavily

glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of

a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including

hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of

other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation

of various immunological functions. Antibodies recognising a common epitope on all of the

isoforms are termed CD45.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                          ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep