For research use only.

Applications: WB

Species Cross-Reactivity: H    

Molecular Weight: ~170KDa

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:1,000

Optimal dilutions should be determined by the end user.

Specificity: The EGFR Mouse Monoclonal antibody detects endogenous EGFR proteins 

Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface

receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.

Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG

and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and

autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits

adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                           ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep