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PCNA Mouse Monoclonal Antibody(12D10)

2014-07-23 19:35:58 点击数:
关键字: XHY044

PCNA Mouse Monoclonal Antibody(12D10)

Catalog

XHY044M

XHY044L

Quantity

50µL

100µL

For research use only.

Applications: WB, IHC

Species Cross-Reactivity: H, R, M

Molecular Weight: 29KD

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1: 5,000, IHC: 1:200

Optimal dilutions should be determined by the end user.

 

Specificity: Antibody can detects endogenous PCNA protein. 

Background: PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the

nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the

processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is

ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants

encoding the same protein have been found for PCNA. Pseudogenes of this gene have been

described on chromosome 4 and on the X chromosome.

 

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                        ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep


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