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β -actin Mouse Monoclonal Antibody(5B7)

2014-07-18 14:06:21 点击数:
关键字: XHY041

 

β -actin Mouse Monoclonal Antibody(5B7)

Catalog

XHY041M

XHY041L

Quantity

50µL

100µL

For research use only.

 

Applications: WB, IHC, IF

Species Cross-Reactivity: H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh

Molecular Weight: 43KD

Isotype: IgG1

 

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1: 5,000, IHC: 1:200, IF: 1:200

Optimal dilutions should be determined by the end user.

 

Specificity: Antibody can detects endogenous β-actin protein 

Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found

in cells of various species and tissues tend to be very similar in their immunological and physical properties.

Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be

noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose

tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

 

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                       ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep

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