For research use only.

Applications: WB

Species Cross-Reactivity: N/A

Molecular Weight: N/A

Isotype: IgG

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000

Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins

Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to

interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as

a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate

recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be

used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different

assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a

Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation   

                          ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep