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Flag-Tag Rabbit Polyclonal Antibody |
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Catalog |
XHY021M |
XHY021L |
Quantity |
50µL |
100µL |
For research use only.
Applications: WB
Species Cross-Reactivity: N/A
Molecular Weight: N/A
Isotype: IgG
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store : -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1:5,000
Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins
Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to
interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as
a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate
recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be
used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different
assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a
Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Applications:WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep