For research use only.

Applications: WB

Species Cross-Reactivity: N/A

Molecular Weight: N/A

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000 

Optimal dilutions should be determined by the end user.

Specificity: The S tag antibody can recognize S tag fusion proteins

Background: S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A).

If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products

remain weakly bound to each other and the protein, called ribonuclease S, remains active although each

of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called

S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity.

This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is:

KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal,

and N-terminal S-tagged proteins

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                        ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep